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This was performed using a precision instrument (Micro Digital Co. Korea).
Thereafter, two 1 mm-sized core biopsies were taken from the morphologically representative areas using a precision instrument (MicroDigital Co., Korea) and then arrayed onto a recipient paraffin block.
Tissue cylinders with a diameter of 1.0 mm were punched from the marked areas of all blocks and brought into a recipient paraffin block using a precision instrument, as previously described [ 38].
Tissue cylinders with a diameter of 1.0 mm were punched from the specimen block and brought into an empty paraffin block [ 8] by using a precision instrument (Beecher Instruments, Silver Spring, MD, USA).
Tissue cylinders of diameter of 1 mm were punched from the marked areas on each block and incorporated into a recipient paraffin block using a precision instrument, the tissue arrayer (Beecher Instruments, Silver Spring, Maryland, USA).
Four cores of 0.6 mm were taken from each donor block and arrayed on a recipient paraffin block using a precision instrument (Tissue Arrayer, Beecher Instruments, Silver Spring, MD, USA).
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Five tissue cores of 0.6-mm diameter in each of the three areas mentioned above were placed into a recipient block by using a precision arraying instrument (Beecher Instruments; Micro Tissue Arrayer, Silver Springs, MD, USA).
Biopsies from the donor blocks were included in recipient tissue array blocks using a precision tissue array instrument (Beecher Instruments, Sun Prairie, WI, USA).
The optrode was advanced to the ventral posterio-medial (VPm) region of the thalamus using a precision microdrive (Knopf Instruments, Tujunga, CA).
The current was continuously monitored using a precision multimeter and a data acquisition system (Keithley Instruments 2700, USA).
A Vitalograph Compact II instrument was used, which was calibrated at least twice daily using a precision syringe.
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