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The powdered tissue was than portioned to 2 mg portions in 2 ml reaction tubes (Sarstedt, Germany) using a precision gauge (R200D, Sartorius, Germany).
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17β- estradiol pellets (0.72 mg, 60-day release; Innovative Research of America, Sarasota, FL) were implanted subcutaneously in the lateral area of the neck using a precision trochar (10 gauge) at the time of cell injection.
Use a precision knife to do this.
The effective diameter of the rotating probe is calibrated by using a high precision grade 0 Mitutoyo gauge block.
All brackets were measured using an electronic gauge (Mitutoyo) and precision pins (Azurea) to obtain a precise mesiodistal bracket (slot) width, slot height, and distance between the two adjacent brackets (Table 1).
Rain rate was measured using a rain gauge.
Pneumatic gauging involves the determination of estimates of dimensional deviations from measurement experiments using a valid gauge model.
One microlitre of either rAAV-NPY+ or rAAV-empty virus (1×109 Pfu/µl) was injected bilaterally over 10 minutes using a 26 gauge guide cannula and a 33 gauge injector (PlasticsOne, Roanoke, USA) connected to a Hamilton Syringe and a syringe infusion pump (World Precision Instruments, Sarasota, Florida).
Samples were sheared using a 20 gauge needle.
Of the 208 biopsies with proof, 199 were performed using a 20 gauge needle, 8 with an 18 gauge needle, and 1 with a 17 gauge needle.
Isolated membranes were circled by using a N27 gauge needle.
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