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One component addressed whole transcriptome analysis of a panel of eight different cell populations isolated directly from 6 mL whole blood, each, collected into EDTA containing tubes using a positive selection with the autoMACS Pro Separator, and of PBMCs prepared from BD CPT (Becton Dickinson Cell Preparation Tubes).
CD14+ monocytes were isolated using a positive selection procedure with magnetic-activated cell sorting according to the manufacturer's protocol (MACS®Miltenyi Biotech, Bergisch Gladbach, Germany).
The number of PHA stimulated PBMCs remained unchanged at six hours and day 2 but increased ∼10-fold at days 5 and 8. cellscells were isolated from fresh monocyte-depleted PBMCs, obtained from 30 mL blood, using a positive selection column following the recommended protocol (EasySep™, StemCell Technologies).
CD4+ T cells were isolated by a mini MACS magnetic sorter (Miltenyi Biotech, UK) using a positive selection protocol according to the manufacturer's instructions.
CD14 monocytes were isolated from patients with RA, using a positive selection procedure with magnetic-activated cell sorting, and labelled with technetium-99m-hexamethylpropylene-amino-oxime.
CD14+ monocytes were isolated from human PBMC by using a positive selection CD14 column according to the manufacturer's protocol (Miltenyi Biotech, Auburn, CA, USA).
Similar(53)
When the nucleotide sequences of the coding regions of all the proteins in this study are used, a positive selection from the ancestor to Pi6C was 1.6216 with a moderate negative selection (Ka/Ks = 0.6399) for the tomato ARPI (See Node 6 in Table S1 and Figure S2).
In this method, we used a positive selection marker flanked by LoxP sites, allowing the marker to be excised by the Cre recombinase.
RMCE was performed using a positive-negative selection strategy previously described (Long et al., 2004).
These experiments confirmed that the sulfonylurea resistance cassette can be used as a positive selection marker for gene deletion in Z. tritici.
Both methods use a positive immunomagnetic selection with anti-epithelial cell adhesion molecule antibodies linked to iron particles to enrich for CTCs.
More suggestions(15)
using a similar selection
using a negative selection
using a particular selection
using a moderate selection
using a small selection
using a single selection
using a clonal selection
using a multilevel selection
using a purposive selection
using a backward selection
using a random selection
using a varied selection
using a specific selection
using a positive deviance
using a positive template
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