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We then addressed the in vitro suppressive activity of Treg using a polyclonal stimulation assay.
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Immunohistochemical analysis was performed by using a polyclonal mouse ascitic fluid at a dilution 1∶750.
A reduction of IgE and IL-6 after TB treatment might also be explained by a reduction of a polyclonal stimulation or the use of anti-tuberculosis drugs, but to our knowledge a beneficial effect by anti-tuberculosis drugs on IgE levels has not been reported.
These assays used a polyclonal antibody against hCG dimer.
We used a polyclonal antibody against Laminin heterotrimer [34].
The kit uses a polyclonal antibody to bind cAMP in a competitive manner.
As loading control we used a polyclonal anti-actin antibody purchased from Santa Cruz Biotechnology Inc (USA).
We used a polyclonal antibody recognizing only the large Cora isoform [35].
To analyze expansion potential and effects of expansion on phenotypic and functional characteristics, CliniMACS8/25 isolated Treg populations were activated using either polyclonal stimulation (anti-CD3+anti-CD28 mAb coated microbeads) or alloantigen stimulation (irradiated PBMC from an HLA-mismatched donor), in the presence of exogenous IL-2.
Treg can be expanded efficiently using polyclonal stimulation [16] [19].
Fibronectin (FN) was detected using a rabbit polyclonal antibody (ab2413; Abcam), and periostin was detected using a goat polyclonal antibody (AF2955; R&D Systems).
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