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Magnetic beads were detached by DETACHaBEAD® CD19, Dynal Biotech (Oslo, Norway) using a polyclonal sheep anti-mouse-Fab antibody.
c-erbB-2 protein expression was investigated immunohistochemically in frozen thyroid tissue specimens from 42 patients using a polyclonal sheep antibody.
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Captured aflibercept was detected using a polyclonal anti-human IgG Fc antibody.
The primary Abs used in this study include a polyclonal rabbit anti-ACE Y1 against pure human kidney ACE (45), a polyclonal sheep anti-ACE HKCE (15) and commercial Abs: anti-CNX Ab from Sigma, anti- PDI mAb, anti-giantin mAb, a Golgi marker from Abcam.
Cells were permeabilized and stained with a polyclonal sheep anti-human-IDO (Pierce Biotechnology, Rockford, IL, USA) and then with FITC-conjugated-rabbit polyclonal anti-sheep antibody (Chemicon, Temecula, CA, USA).
5-BrdU was detected using a sheep polyclonal antibody (Maine Biotechnology Services Inc., Portland, ME, USA).
To purify endogenous telomerase from HeLa cells and HeLa cell lines overexpressing mCherry-hTERT variants, telomerase was immuno-purified using a sheep polyclonal antibody (a kind gift from S Cohen) as previously described (Cohen and Reddel, 2008).
These assays used a polyclonal antibody against hCG dimer.
We used a polyclonal antibody against Laminin heterotrimer [34].
Comparable results were observed in the absorbance readings from ELISAs using a commercially purchased sheep anti-SEB polyclonal antibody).
Specificity ELISA used polyclonal sheep anti-mouse IgG Ab (The Binding Site, Birmingham, UK) to detect the binding of patient-specific or non-specific control anti-sera to each protein.
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