Sentence examples for using a polyclonal serum from inspiring English sources
Toxoplasma gondii IHC was carried out on samples from Cases 1 and 2 (the five aforementioned brain areas, along with the H&E-stained sections of all tissues in which coccidian parasites were identified by histopathology and/or biomolecular tests)25, using a polyclonal serum of caprine origin (VMRD).
Tubulin was detected using a monoclonal antibody (Sigma, 1∶2000) and endogenous TPI was detected by using a polyclonal serum ([32], 1∶5000).
The successful IHC demonstration of ABV antigens in birds with PDD by using a polyclonal serum against the phosphoprotein of BDV shows that, despite their diversity, ABVs and BDV share epitopes.
As the monoclonal antibody is raised against an epitope at the C-terminus of type VII collagen, and the RDEB patients investigated here suffer from C-terminally typecated type VII collagen proteins, we additionally analyzed the expression of type VII collagen protein by immunofluorescence microscopy using a polyclonal serum.
To further explore this possibility, we prepared spot slides from the cellular fraction of the throat wash and examined them with an indirect immunofluorescence assay by using a polyclonal serum from a rabbit immunized with a recombinant nucleocapsid protein of SARS-CoV.
To verify the gene replacement of the TPI1 coding sequence, yeast clones exhibiting no growth on media supplemented with glucose as carbon source have been selected and the correct insertion of the DNA fragment was confirmed by PCR and by western blot analysis using a polyclonal TPI serum (1∶4000, [32]).
Western blotting: samples were subjected to reducing 15% mini SDS-PAGE and analysed by Western blotting using a polyclonal rabbit serum anti-human IL-1Ra and a goat anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase, as described in detail elsewhere [ 48].
In this study, the immunohistochemical expression of a new inducible elastase inhibitor, SKALP (skin-derived anti-leucoproteinase)/elafin, in the tissue of squamous cell carcinoma and uninvolved oesophageal mucosa was studied using a polyclonal rabbit anti-serum against SKALP/elafin.
Plates were fixed and dried, then stained with immunoperoxidase as described by Meyling [ 18], using a polyclonal bovine anti-BVDV serum (BVD virus positive control serum, VLA, Weybridge, UK) to detect the virus.
In Figure 4, the presence of His-Tag fusion protein was detected by anti-HisG antibody which recognizes the sequence -His-His-His-His-His-His-Gly (6 × His-Gly epitope) on the fusion protein while the antigenicity of the expressed protein was further detected by using a polyclonal positive dengue IgG serum in Figure 5 which detects the prM target protein.
To exclude the possibility of other subtle inactivating mutations, immunoblot analysis of protein lysates was performed using a polyclonal anti-p16INK4 rabbit anti-serum.
