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Fractions (1 mL each) were collected, washed and analyzed by immunoblot using a polyclonal rabbit anti-IscU antibody [71], [72].
Using a polyclonal rabbit antibody against the synthetic peptide EQPT, expression of SPANX-N proteins was examined in nongametogenic tissues.
Furthermore, we performed western blot analysis using a polyclonal rabbit anti-lysozyme antibody to confirm the production of lysozyme in immature eggs and oviposited eggs.
The immunohistochemical analysis was performed by the indirect immunoperoxidase method has described elsewhere [39] and using a polyclonal rabbit antibody against R. conorii as primary antibody.
HSLbeta was selectively detected using a polyclonal rabbit antibody raised against the exon A-encoded sequence of HSLbeta [5], and for HSLtes a polyclonal antibody against exon T-derived sequences was used [27].
The membranes were probed for Fas ligand using a polyclonal rabbit anti-Fas ligand antibody [24] followed by a goat anti-rabbit-HRP secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA).
Similar(10)
Next, MFN2 was detected by using a polyclonal rabbit-anti-MFN2 (N-terminal) serum (Sigma), a goat anti-rabbit IgG-HRP conjugate (DAKO) as secondary antibody and a chemiluminescence-based detection system (ECL plus, GE healthcare).
For phospho-Src, we used a polyclonal rabbit anti-human antibody (1 : 1000; Sigma-Aldrich).
For CycD1, we used a polyclonal rabbit anti-human antibody (1 : 500; Sigma-Aldrich).
For MMP-9, we used a polyclonal rabbit anti-human antibody (1 : 1000; Sigma-Aldrich, Saint Louis, MO, USA).
For Maspin, we used a polyclonal rabbit anti-human antibody (1 : 1000; Sigma-Aldrich).
More suggestions(15)
using a polyclonal goat
using a polyclonal guinea-pig
using a polyclonal sheep
using a secondary rabbit
using a tame rabbit
using a polyclonal antiserum
using a polyclonal phosphovimentin- pS56 -Ab
using a phosphospecific rabbit
using a normal rabbit
using a polyclonal antibody
using a polyclonal radioimmunoassay
using a commercial rabbit
using a polyclonal guinea
using a dummy rabbit
using a polyclonal stimulation
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