Sentence examples for using a polyclonal goat from inspiring English sources

NKp46 staining was made using a polyclonal goat antihuman NKp46 antibody, concentration 4 µg/ml, (R&D Systems, Lille, France).

Our laboratory has also reported the presence of surface LAP+ on mouse T cells using a polyclonal goat anti-human LAP antibody [4], [5].

5 10×106 cells in [DMEM, 10 mM HEPES (pH 7.5)] were incubated on ice with 20 µg/ml anti-CD3ε and 10 µg/ml anti-CD28 mAb (BD Pharmigen; Cat. #145-2c11 and #37.51, respectively) for 30 or 60 min, followed by cross-linking for 5 min at 37°C using a polyclonal goat anti-hamster Ab (Caltag Laboratories, Burlingame, CA; Cat. #HA6101).

WB analysis was performed using a polyclonal goat anti-ApoE (Millipore) and a polyclonal goat anti-Mertk (R&D) as previously described (Houssier et al, 2008).

tPA immunoblottings were performed using a polyclonal goat antibody (Santa Cruz Biotechnology – sc5239; 1 : 1000, Heidelberg, Germany) followed by incubation with the appropriate peroxidase-conjugated secondary antibody.

Levels of ANT protein were determined using a polyclonal goat anti-ANT antibody (Santa Cruz Biotechnology, CA, USA) as described previously [ 29].

We used a polyclonal goat antiserum (Chemicon, Temecula, CA; product number AB144P) prepared against human placental ChAT that was affinity-purified.

For detection of ANGPTL4 we used a polyclonal goat anti-human antibody against the N-terminal domain (N-15, Santa Cruz Biotechnology), a goat polyclonal anti-mouse against the C-terminal domain (L-17, Santa Cruz Biotechnology) and a rabbit polyclonal antibody against full-length ANGPTL4 from Abcam, Cambridge, UK.

Binding of a recombinant human NKG2D-Fc fusion protein (139-NK, R&D Systems) to MICA-expressing cells (0.2 μg/10 cells in 100 μl PBS) was assessed using a polyclonal FITC-conjugated goat anti-human IgG Ab as secondary reagent.

Chemiluminescent reagents (Pierce; Rockford, IL) and the following antibodies were used for detection; a polyclonal goat anti-human galectin-9 antibody (R&D Systems) used at 1∶1000, followed by a donkey anti-goat HRP secondary (R&D Systems).

Importantly, this higher molecular weight band was absent when a polyclonal goat anti-mouse C3 antibody was used in place of the polyclonal goat anti-mouse FD during immunoprecipitation (Fig. 3b).