Sentence examples for using a polyclonal antiserum from inspiring English sources

Using a polyclonal antiserum against VAMP7 [ 39], similar results were found (results not shown).

Using a polyclonal antiserum we also proofed presence of Gd protein in endometrial tissue.

Silencing was confirmed by western blot analysis using a polyclonal antiserum directed against recombinant OPR3 expressed in E. coli [ 40].

SLC22A1 immunohistochemistry was performed using a polyclonal antiserum raised in rabbits against a synthetic peptide corresponding to the carboxy-terminal sequence of human SLC22A1 as described previously [ 20].

Serial frozen sections were used to localise VEGF by indirect immunoflourescence method using a polyclonal antiserum made against recombinant human VEGF165 (Olson et al, 1996).

We next examined two series of human breast cancer patients for tumoral expression of syncytin-1 using a polyclonal antiserum raised to a synthetic nonapeptide derived from the syncytin-1 sequence.

In this study, we have used a polyclonal antiserum against Pea-PVK-2, which specifically recognizes the C-terminus PRXa ([39]) shared by the CAPA and HUGIN peptides, as well as marker molecule expression driven by Va-GAL4 (see below, [40]) and hug-GAL4 [35].

To examine whether there was a lack of α-Tectorin protein in the rst mutant, we used a polyclonal antiserum raised against the von Willebrand domain (VWD) repeats of the chick α-tectorin protein (Knipper et al., 2001).

At the end of 2-AAF administration, animals were sacrificed and the content of 2-AAF-adducts in liver DNA was determined by enzyme-linked immunoadsorbent assay using a polyclonal rabbit antiserum.

To test this further, we performed Western-blot analysis of control and Sans mutant ovaries using a polyclonal anti-Sans antiserum raised against the full-length protein (see Materials and Methods).

Finally, bound serum proteins were eluted with 100 mM glycine-HCl (pH 2.0) for 15 min and all supernatants collected (last wash fraction, fractions from the incubation reactions with increasing salt concentrations, and the eluate fraction) were then analyzed by Western blotting using a polyclonal anti-CFHR1 antiserum as described above.