Sentence examples for using a policeman from inspiring English sources

For this assay, the medium was removed and the cells resuspended in 1.5 ml of 10% sucrose in PBS, using a policeman.

For the western blot assay cell lysates of hMVEC were prepared by scraping the wells on ice using a rubber policeman and by adding Roche lysis buffer with phosphatase and protease inhibitors (Roche, Mannheim, Germany).

Cells were washed twice with ice-cold phosphate-buffered saline (PBS) and thereafter collected using a rubber policeman.

Whole cellular extracts from control and stimulated cells were prepared from cells detached using a rubber policeman, and harvested from culture medium into Eppendorf tubes.

Cells were washed three times in cold PBS containing 100 µg/ml cycloheximide and were scraped off the plate using a rubber policeman and 1 ml of the same solution.

Cells were scraped using a rubber policeman before addition of another 50 μl lysis buffer.

Scrape the lysed cells from the dish using a rubber policeman.

Cell lysates were collected from culture plates using a rubber policeman, and protein collected by centrifugation.

Any remaining cells were detached using a rubber policeman, washed once with PBS, and collected by centrifugation.

In a reciprocal approach, mesangial cells were grown to confluency and a scratch was introduced using a rubber policeman.

The cells were incubated for 30 minutes at 4°C on a shaker and then harvested using a rubber policeman.