Sentence examples for using a point mutation from inspiring English sources

Plasmid DNAs, bearing two Lac repressor binding sites spaced at a designed distance, are created using a point mutation method (QuikChange site-directed mutagenesis, Stratagene) on plasmid pUC19.

Using a point mutation of GCC from GAC (wild-type), 3'–5' exonuclease activity deficient mice were created by D400A exchange, which is located in the exonuclease active site (ExoII) and with which mice suffered from higher tumor susceptibility [16], [17].

The interaction between the miRNAs and the target mRNA sequences was further validated using a point mutation of the putative miRNA-binding site in the 3′ UTR of the FKBP51 gene.

Finally, we used a point mutation discovered during biophysical investigations of Ci-VSP to design ArcLightning, a novel version of ArcLight with accelerated movements of the voltage-sensing domain and improved fluorescence response times.

The authors then validate their structure through multiple approaches, including the rescue of mutants in the C-lobe through mutation of opposing residues in the N-lobe, and using a point mutant equivalent to a key mutation in Angelman's Syndrome, they underscore the medical relevance of their studies.

The ENU-induced mutant strain we used carried a point mutation at the Gdf5 locus.

This mechanism was discovered by Bultman et al. using a hypomorph point mutation (E1083G) located in the ATPase domain of SMARCA4.

For construction of double mutants we used pHT315Ab-D136N harboring a point mutation D136N as template to introduce additional point mutations as E129K or T143D.

Site-directed mutagenesis was used to introduce a point mutation at −774 bp of the putative β-Syn promoter, specifically within a metal response element (MRE).

Site-directed mutagenesis was used to construct a point mutation at the equivalent conserved CTPsyn residue (CTPsynE160K) in CTPsyn-YFP expression vector (supplementary material Fig. S2).

More recently, the mechanism of sulphonylureas on KATP channel activation by Mg nts was examined in more detail using KATP channels with a point mutation G334D in the Kir6.2 subunit, Kir6.2 G334D/SUR1 channels [ 30].