Sentence examples for using a point mutant from inspiring English sources
For this method, the idea was to occlude access to the TEVp active site using a point mutant of the FKBP12 protein termed Fm [24].
The authors then validate their structure through multiple approaches, including the rescue of mutants in the C-lobe through mutation of opposing residues in the N-lobe, and using a point mutant equivalent to a key mutation in Angelman's Syndrome, they underscore the medical relevance of their studies.
To directly test the possibility that neuroprotection by SIRT1 was not dependent on its catalytic activity, we used a point-mutant form of SIRT1-H363Y whish is catalytically inactive [14], [40], [57].
Our recent study has showed that HIF-1α could promote osteogenesis of bone mesenchymal stem cells (BMSCs) using a gene point mutant technique.
To test whether EpCAM needs to be present at the cell surface to allow CTF generation, we used an EpCAM point mutant (C66T) that causes CTE [ 8].
Furthermore, a point mutant coding for an inactive Dohh-E57A (Dohhmut) mutant (Kang et al., 2007) was generated using the Phusion site-directed mutagenesis kit (Finnzymes, Vantaa, FIN).
Using alanine point mutants of GB1, we show that the amplitude of mechanical stability enhancement of GB1 by Fc does not correlate with the binding affinity, suggesting that binding affinity only affects the population of GB1/human Fc (hFc) complex at a given concentration of hFc, but does not affect the intrinsic mechanical stability of the GB1/hFc complex.
The importance of the SvID was further tested using menin point mutants, which were reported in MEN1 patients.
Various studies using alanine point mutants and synthetic peptide scaffolds have collectively reported the importance of amino acid residues Q170, K171, E172, G173, W190, T195, and L196 of CCR5 EC2 in 2D7 recognition.
A Φ-value analysis was then undertaken for HT PagP using point mutants of 19 residues spread throughout the protein structure [157].
