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Absorbance at 550 nm and 650 nm (reference wavelength) was measured using a plate spectrophotometer.
Absorbance was then read at 490 nm by using a plate spectrophotometer.
The absorbance values were read using a plate spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) at 450 nm.
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Subsequently, the absorption of purple formazan was recorded at 570 nm using a plate reading spectrophotometer to quantify the percentage of cell viability.
The optical density at 540 nm was then measured using a plate reading spectrophotometer (Dynex Technologies, MRX II).
Absorbance was measured at 595 nm by using a plate reader spectrophotometer (Tecan).
The kinetics of ONPG hydrolysis were measured by determining the absorbance at 400 nm using a plate reader spectrophotometer.
For this, cells were incubated for 1 hour with WST-1 reagent, and cell viability was determined by quantification of the absorbance at 440 nm by using a plate reader spectrophotometer (Tecan, Grödig, Austria).
Fluorescence intensity data was gathered at 10-second intervals using a plate reading fluorescence spectrophotometer (Molecular Devices) with an excitation wavelength of 589 nm and emission wavelength of 617 nm.
Cell growth was monitored by culture absorbance (620 nm) using a plate-reading spectrophotometer (Biotek PowerWave XS; Biotek, Winooski, VT, USA).
To test for effects of p53 and Toca-1 silencing on cell viability, cells (5,000) were seeded in 96-well plates in triplicate for 18 hours, and analyzed following addition of AlamarBlue™ for 5 hours using a plate-reading spectrophotometer to measure absorbance at 570 nm.
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