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Subsequently, the absorption of purple formazan was recorded at 570 nm using a plate reading spectrophotometer to quantify the percentage of cell viability.
Fluorescence intensity data was gathered at 10-second intervals using a plate reading fluorescence spectrophotometer (Molecular Devices) with an excitation wavelength of 589 nm and emission wavelength of 617 nm.
After washing, the plates were incubated with streptavidin-HRP, developed on an appropriate substrate, and OD450 was determined using a plate reading immunoadsorption enzyme.
The optical density at 540 nm was then measured using a plate reading spectrophotometer (Dynex Technologies, MRX II).
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To each well 100 µL TMB substrate (3, 3', 5, 5'-tetramethylbenzidine, Pierce Biotechnology) was added and OD 650) absorbance readings were acquired for each well at 10 second intervals using a plate-reading UV/Vis spectrophotometer (Molecular Devices).
After incubation for 10 minutes at room temperature luminescence was measured using a plate-reading luminometer.
Cells were incubated at 37°c for 96 hrs, and fluorescence was subsequently monitored using a plate-reading fluorometer.
The plate was incubated at room temperature for 3 h prior to quantification using a plate-reading luminometer (SpectraMax i3, Molecular Devices).
Cells were processed for analysis of HRE-luciferase activity as described (Dual-Luciferase Reporter Assay System, Promega) using a plate-reading luminometer (Victor, Perkin Elmer, Milano, Italy).
Following incubation for 30 min–3 h, luminescence of each sample was read using a plate-reading luminometer as directed by the manufacturer.
Cell growth was monitored by culture absorbance (620 nm) using a plate-reading spectrophotometer (Biotek PowerWave XS; Biotek, Winooski, VT, USA).
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