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Absorbance was measured using a plate reader (VersaMax, Molecular Devices, US) at 540 nm wavelength.
The A450 value was measured using a plate reader.
The resulting luminescence was measured using a plate reader (SpectroFluor Plus, Tecan).
Anthocyanin content was quantified using a plate reader (SpectraMax 190- Molecular Devices).
Stain intensity was quantified by spectrophotometry at 570 nm using a plate reader.
The absorbance in each well was measured at 450 and 630 nm using a plate reader.
The optical density (OD) at 414 nm of each well was measured using a plate reader.
Finally, the plates were analyzed by measuring absorbance at 450 nm (reference at 620 nm) using a plate reader.
Alizarin red staining was semiquantitatively analyzed at λ595 nm using a plate reader (Bio-TEK-instruments Inc., Winooski, VT, USA).
The plates were incubated for 15 min at room temperature and read at 570 nm using a plate reader.
Medium was aspirated, formazide crystals were disolved in DMSO, and absorbance was measured at 495 nm using a plate reader.
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