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Twenty brine shrimp larvae were placed in vials containing extract using a plastic pipette with a 2 mm diameter tip.
Then, the cell layer was gently wounded using a plastic pipette tip (P200) and rinsed with PBS before the culture medium was replaced.
A scratch wound was made the next day using a plastic pipette tip.
Once cells reached 100% confluency, a wound was created in the cell monolayer using a plastic pipette tip.
Cell lines 1542-NPTXX, 1542-CPC3X, PC3 and DU145) were grown on glass coverslips to confluency and scratched using a plastic pipette tip.
The larva was poked gently on the abdomen using a plastic pipette to mimic a predatory attack.
Similar(50)
For single sphere passaging, individual 200 250 µm diameter spheres were selected, mechanically dissociated using a plastic P200 pipette tip and plated in a single well of a 24-well plate.
The virus band was harvested using a plastic Pasteur pipette and centrifuged 3 × through Amicon 100 kDa filter (Ultra-4 Cat. No. UFC810024), resuspending each time to maximum load volume with TEN.
Epithelial cells were collected at the 25 40% Percoll interface using a plastic disposable Pasteur pipette (Copan, Brescia, Italy).
Once sedated, the vocal chords were visualized using an operating otoscope (Welch Allyn) and 30 μL of bacteria or PBS was instilled into the trachea during inspiration using a plastic gel loading pipette tip.
Eggs were then transferred to a 4-mL Beckman Ultra-Clear centrifuge tube (Beckman 344062), using a disposable plastic pipette with the tip cut off, taking care to make sure the eggs were not exposed to air.
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