Sentence examples for using a plastic pestle from inspiring English sources

Individual guts were dissected under aseptic conditions and homogenized in 100 μl sterile PBS using a plastic pestle.

Leaf discs were homogenised using a plastic pestle in a 1.5 ml microcentrifuge tube and incubated with 300 μl of the appropriate extraction buffer.

Total RNA from frozen leaf material was extracted by homogenizing material in Plant RNA Reagent according to instructions (Invitrogen, Carlsbad, USA) using a plastic pestle.

5-10 eggs/embryor or 3-5 larvae were pooled and homogenized in 1.5 ml microcentrifuge tubes containing lysis buffer (Qiagen RNeasy mini kit) using a plastic pestle.

One half of the muscle was transferred to a microfuge tube and homogenized by hand in the lysis/binding solution supplied with the kit using a plastic pestle.

Larvae were then weighed, dipped in 70% ethanol to kill surface contaminants, and homogenised in 500 μl M9 minimal medium using a plastic pestle.

This precooled buffer was added directly on ice to the frozen slices in Eppendorf tubes and the tissue was mechanically homogenized using a plastic pellet pestle.

The pellet was washed once in lysis buffer A and resuspended into 200 μL of buffer B (10 mM Tris-HCl, 1% w/v SDS, 5 mM EDTA-NA2, 0.15 mM desferroxamine, pH 8.0) using a plastic Kontes Pellet Pestle®.

RNA was isolated from a single ganglion homogenized in a 1.5 mL tube with use of a plastic pestle and lysis buffer and purified by use of the RNeasy mini kit (Qiagen).

The tissue sampled was combined with 100 200 ul of autoclaved 1×PBS in a sterile 1.5 ml microcentrifuge tube and homogenized using a sterile plastic pestle.

Each sample was homogenized using a clean plastic pestle, placed on ice for 30 min, and centrifuged at 14,000 g for 5 min. The supernatant was then transferred from each tube to a separate 0.5 ml microcentrifuge tube and frozen at −20°C until further analysis.