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The efficacy of silver nanoparticles and AgNO3 was determined using a plaque reduction assay similar to previously described methods [12].
Nanoparticles (10 80 nm, with or without polysaccharide coating), or silver nitrate (AgNO3) at concentrations of 100, 50, 25, and 12.5 μg/mL were evaluated for efficacy using a plaque reduction assay.
Lung virus titers were determined using a plaque assay [29], [30].
Virus blood titers were measured using a plaque forming assay as described [14].
Finally, the selected candidates were subjected to a biological validation assay to assess inhibition of Dengue virus propagation in mammalian host cells using a plaque formation assay.
The EC50 value obtained for SPL7013 in our assay was similar to the value obtained using a plaque reduction assay for HSV [24].
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Viral loads were determined using a plaque-forming cell assay.
Virus titers were measured using a plaque-forming assay as described previously.
We measured the ability of IVIg preparations to inhibit influenza subtype H5N1 replication by using a plaque-reduction assay.
The enrichment of tumor-associated proteins after biopanning was tested using a plaque-lift assay and immunochemical detection.
Using a plaque-reduction neutralization test (PRNT), we confirmed infection in 2 of these patients and showed cross-reactivity of antibodies among the 3 virus strains.
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