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The historical effects of natural selection on the cytb protein were estimated with the software TreeSAAP using a phylogenetic reconstruction for 107 mammal taxa from all major mammalian lineages using 13 complete protein-coding mitochondrial gene sequences.
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sinensis and C he. mydas, we used a phylogenetic reconstruction of the beta-keratin genes from the three turtles.
All 152 I. ricinus Kunitz peptide sequences were used for a phylogenetic reconstruction of the Kunitz-domain family within ticks.
The 12 Pristionchus cellulase ESTs that contained the carbohydrate-binding module at their carboxyl terminus were used in a phylogenetic reconstruction of the genes' history.
The evolutionary history of S4 was analyzed using a ML phylogenetic reconstruction of all the sequences of r-protein S4 and its paralogs obtained from the 660 genomes, as described in Methods. Figure 3 shows an unrooted phylogenetic tree obtained from a consensus of 1000 ML trees.
A phylogenetic reconstruction using presence or absence data of genes in the pan genome was performed using the maximum parsimony method in PAUP* version 4.010b [ 41].
To gain insight into the natural history of Bacillus based on the complete genome sequence, we performed a phylogenetic reconstruction using all of the core genes.
Sturmbauer et al. [ 27] defined 12 mitochondrial lineages based on a phylogenetic reconstruction using a parsimony method implemented in TCS [ 31], which have evolved in the course of two major radiations about 700 945 Ka and 399 540 Ka.
Figure 2 shows the results of a phylogenetic reconstruction using maximum likelihood analysis, which classifies the full-length zebrafish OlfC genes into monophyletic clades representing 17 subfamilies showing on average 46% inter-subfamily identity.
Previous work [ 27] combined mtDNA control region sequences of 462 Tropheus samples into a neighbour joining (NJ) tree, and classified samples into mitochondrial lineages corresponding to unconnected networks resolved by a phylogenetic reconstruction using a parsimony method implemented in TCS (ver. 1.13 [ 31]).
Therefore, a phylogenetic reconstruction was used (additional file 6).
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