Exact(4)
Radioactivity of the 45S pre-rRNA was measured after exposure of the membranes using a PhosphorImager system (Typhoon and ImageQuant TL, Amersham).
The dried gel was analysed using a phosphorimager system.
Images were acquired using a phosphorimager system FLA5100 (Fuji Photo Film Co., Tokyo, Japan).
Once destained, gels were dried using an ATTO RapiDry system (ATTO, Tokyo, Japan), and P-labelled proteins were then detected using a phosphorimager system (Molecular Dynamics, Sevenoaks, UK).
Similar(56)
The data were analyzed using a PhosphorImager (Bio-Rad).
The UV of the standard was identified using a UV lamp, while the movement and integration of the radioactive spots were visualised and measured using a phosphorimager (BAS 2500 Phosphorimager, Fujifilm, Tokyo, Japan) with Fujifilm Multigage 3.0 software.
Following autoradiography using a phosphorimager screen, results were analyzed using Quantity One software (Bio-Rad).
Radioactive bands were visualized by radiography using a phosphorImager after overnight exposure on a phosphor screen.
Samples were run on 20% denaturing polyacrylamide gels and visualized using a PhosphorImager.
Bands corresponding to substrate and products were quantified using a phosphorimager.
Phosphatidylinositol 3-kinase activity was determined by autoradiography and quantified using a Phosphorimager (Amersham Biosciences, NJ).
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