Sentence examples for using a peroxidase substrate from inspiring English sources

Slides were then incubated with a peroxidase-conjugated rabbit anti-guinea pig IgG (Dako, Ely, UK) and stained using a peroxidase substrate kit (DAB, Vector Laboratories, Peterborough, UK).

The amount of bound detector antibody was determined after 1 h incubation with the peroxidase-conjugated streptavidin (Pierce) using a peroxidase substrate orthophenylene diamine (Sigma).

After washing with PBS three times for 5.0 min each time, the immune complexes were visualized using a Peroxidase Substrate DAB kit (Gene Tech Co., Ltd., Shanghai, China) according to the manufacturer's instructions.

The transblotted membrane was blocked for 1 h with a blocking buffer (4 % (w/v) BSA in PBS, pH 7.4) and then incubated with anti-E-tag antibody HRP conjugated (1∶2000) for 2 h at RT. 4-CN (4-chloro-1-naphthol, Sigma) was used as a peroxidase substrate to visualize the immunoreactivity.

Liquid DAB (3,3'-diaminobenzidine) substrate chromogen system (Dako) was used as a peroxidase substrate.

Staining was visualized using diaminobenzidine containing a peroxidase substrate (DakoCytomation).

After extensive washing in PBT, the peroxidase reaction was carried out using a DAB peroxidase substrate kit (Vector Laboratories), and the embryos were mounted in 70% glycerol and dissected.

At the end of the western-blot protocol, the specific signal was probed using a Horseradish peroxidase substrate (Millipore).

Bound antibodies against PA were detected by using a chromogenic peroxidase substrate (tetramethylbenzidine).

The ELISA procedure for the detection with HisProbe™-HRP (Thermo Scientific, Rockford, USA) was done according to the manufacturer's instructions using ABTS (Sigma) as a peroxidase substrate.

The target protein was visualised by incubation for 30 min with a peroxidase substrate, using diaminobenzidine tetrahydrochloride (DAB) as the chromogen.