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The animals were deeply anesthetized with pentobarbitone (0.05 mg g−1) and perfused via the heart, using a perfusion pump, at a speed of 7 ml/min with 2.5% glutaradehyde, and 2% paraformaldehyde, in phosphate buffer (0.1 M, pH 7.4, 250 300 ml per animal).
For patch-clamp experiments, slices were transferred to the recording chamber perfused with ACSF at a rate of 1 ml/min using a perfusion pump 2232 Microperpex S (LK.B, Upsalla, Sweden).
A flow of diluted Sodium Hyaluronate (also known as Hyaluronic Acid, (HA)) is set up from the RW to the SV opening using a perfusion pump that sets up a unidirectional flow.
using a perfusion pump (Harvard Instruments, Edenbridge, UK), 0.3 ml/min [ 13].
E2 and XEs were administered using a perfusion pump system at a rate of 2 mL/min.
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We used a perfusion device for the renewal or exchange of the culture media, consisting of 2 Teflon tubes (Tygon, Fisher Bioblock SA) and of a peristaltic pump (Ismatec).
After CRS four intraabdominal drains and two temperature probes are placed for continuous abdominal perfusion using a roller pump system with heat exchanger as described before [ 72].
Perfusion was accomplished by inserting a 25 G needle into the inferior vena cava, cutting the portal vein, and using a peristaltic pump to deliver perfusion solutions.
The test chemicals were placed in syringes on a five-valve manifold and added into the perfusion chamber using a micro pump with a flow rate of 200 μl per min. The application lengths of Echinacea extracts, fractions and chemicals were indicated by the bars on the graph as well as in the figure legends.
Hindlimbs were continuously perfused, using a peristaltic pump, with warmed (37°C), oxygenated Krebs-Henseleit solution (pH 7.4) containing dextran, 40 g/l, at a flow rate that produced a perfusion pressure of 80 mmHg.
Other limitations were the small number of animals per group and the short experimental duration of ex-vivo perfusion using a centrifugal blood pump providing non-pulsatile flow.
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