Sentence examples for using a perfusion culture from inspiring English sources
Using a perfusion culture system with conditions closer to the in vivo situation, cells proliferated and differentiated on iron foams coated with either brushite or HA while in static cell culture cells could proliferate only on Fe-brushite.
To maintain the desired temperature of 37°C within a perfusion culture container a heating plate (MEDAX-Nagel, Kiel, Germany) and a cover lid (not shown) is used (Figure2d).
To minimize the content of gas bubbles within a perfusion culture set-up, a gas expander module was developed[36].
When a perfusion culture experiment is performed, the pH must be adjusted and sufficient respiratory gas must be present.
The tissue carrier was transferred to a perfusion culture container with horizontal flow characteristics.
We used a perfusion device for the renewal or exchange of the culture media, consisting of 2 Teflon tubes (Tygon, Fisher Bioblock SA) and of a peristaltic pump (Ismatec).
If such a formulated medium is used for perfusion culture outside a CO2 incubator, the pH will shift from the physiological range to much more alkaline values due to the low content of CO2 (0,3%) in atmospheric air.
For that reason any medium used for perfusion culture outside a CO2 incubator has to be stabilized by reducing the NaHCO3 concentration and/or by adding biological buffers such as HEPES (GIBCO/Invitrogen, Karlsruhe, Germany) or BUFFER ALL (Sigma-Aldrich-Chemie, München, Germany).
In order to explore the feasibility of employing 2P endoscopy for ex vivo study of the aqueous outflow pathway using an anterior segment perfusion culture system of the eye, we constructed a light-weight, catheter-like microscope and demonstrated its capability for imaging human TM.
We use the perfusion culture system to produce FSS exerting on human bone marrow-derived mesenchymal stem cells (hMSCs) and thus activate ERK1/2.
The system was used for a preliminary perfusion culture experiment using E14 mouse embryonic stem cells.
