Sentence examples for using a percoll gradient from inspiring English sources

Liver and lung mononuclear cells were isolated using a Percoll gradient.

For flow sorting late schizont stage parasites were separated from uninfected RBCs using a Percoll gradient.

Eosinophils were purified using a percoll gradient followed by immunomagnetic cell separator.

The cytoplasm and nuclei were extracted using a Percoll gradient centrifugation adapted from previous methods.

Peripheral blood leucocytes were isolated from the venous blood of D.p.-specific IgE-positive asthmatics using a Percoll gradient solution.

The mating mixture was digested with glusulase and spores were purified using a Percoll gradient as described (Sun et al. 2013).

Different erythrocytes subpopulations were isolated using a Percoll discontinuous gradient [9], [10], [11].

A mixed fraction of N. caninum organelles was purified using a Percoll density gradient essentially as described for T. gondii [24].

Briefly, the method involves mild sonication of the cells followed by a low-speed centrifugation to remove cell debris, and a final purification of the granules using a Percoll density gradient.

Briefly, perfused organs were minced, incubated with HBSS+ 1.3 mM EDTA solution for 30 minutes at 37°C, re-suspended in 225 U/ml type I collagenase for 60 minutes at 37°C, and cells were centrifuged using a Percoll density gradient to isolate lymphocytes from the parenchyma.

Peripheral blood mononuclear cells were extracted from whole blood of uninfected cattle using a Percoll™ gradient (GE Healthcare UK, Buckinghamshire, UK) and previously described methods [ 49].