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The reaction mixture was filtered using a peptide vessel, concentrated and the peptide was precipitated by the addition of ether.
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We next addressed whether cleaved amyloid β peptides localize similarly to APP using a peptide-specific antibody.
The resin (1 equiv) was swollen in DMF (10 mL) for 2 min in a peptide vessel.
Previously published peptide transport assays tend to follow peptide uptake using a radiolabeled peptide substrate.
Each 30 μg peptide mixture was manually loaded onto separate columns using a pressure vessel.
"One of the Coast Guard's excuses for not using a lease vessel, or a less expensive vessel, is that it's not a military-type vessel," Hunter said.
These antibodies were generated in sheep using a nested peptide series conjugated to the metalloprotein, KLH.
Characterization of antibodies against histone PTMs used in the study was performed using a Histone Peptide Array (Active Motif, 13005).
Characterization of prorenin activation using a synthetic peptide substrate.
Peptides were synthesized using an automated peptide synthesizer (MBS 396; Advanced Chemtech, Louisville, USA) using fluorenylmethoxycarbonyl chemistry.
Loss of endogenous mouse β-cells was confirmed by measuring serum mouse C-peptide using a Mouse C-peptide ELISA (ALPCO Diagnostics; 80-CPTMS-E01 80-CPTMS-E01 80-CPTMS-E01
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