Sentence examples for using a peptide substrate from inspiring English sources

When tested in an in vitro PDK1 enzymatic assay using a peptide substrate, the benzodiazepin-2-ones increased the activity of the enzyme in a concentration-dependent fashion, indicating these compounds act as PDK1 allosteric activators.

We then measured the activity of these samples using a peptide substrate (syntide) and a continuous enzyme-coupled assay (ADP Quest, see 'Materials and methods').

Similarly, a much larger relative stimulation of PKN1 over PKN3 by arachidonic acid was previously reported using a peptide substrate based on the PKCδ pseudosubstrate sequence [ 34].

SH-SY5Y cells were transfected with wild-type α-synuclein for 72 h, and proteasome activity in cell lysates was measured using a peptide substrate specific for proteasome, zLLE-MCA.

Using MBP as substrate, we were able to obtain IC50 values for Staurosporine of 11.5 nM, which were very similar to those obtained using a peptide substrate (19 nM, not shown).

The kinase activity of the CaMKII-Hcp1 fusion was tested using a peptide substrate (syntide) and it displayed cooperative activation by Ca2+/CaM, with an activation profile similar to that of wild-type CaMKII (Gaertner et al., 2004; Rosenberg et al., 2005, data not shown).

Previously published peptide transport assays tend to follow peptide uptake using a radiolabeled peptide substrate.

We next verified that our finding of an Arg8-dependent PCAF activity was not an artifact stemming from the use of a peptide substrate but also operated for protein and nucleosome substrates.

We also compare the sensitivity of this method to that using a fluorogenic peptide substrate, carbobenzoxy-LRGG-7-amido-4-methylcoumarin (Cbz-LRGG-AMC), that has also been used as a substrate for DUBs (19).

Protease assay was performed using a fluorogenic peptide substrate and measured by fluorescence spectrometry.

We also tested the effect of autophosphorylation on enzymatic activity, using a synthetic peptide substrate.