Sentence examples for using a peptide sequencer from inspiring English sources

The purified β-mannanase protein was electroblotted onto the transfer membrane, stained by Ponceau S (Nacalai, Kyoto, Japan), and analyzed using a peptide sequencer (Procise 492-HT Protein Sequencer, Applied Biosystems).

N-terminal amino acid sequence analysis (A-T-T-G-I-H-V-A-N-G) using a peptide sequencer indicates that the recombinant β-mannanase had a signal peptide from M1 to A37, and the sequence between A37 and A38 was cleaved during the secretory process.

We thank Dr. Satoshi Kawamoto for analyzing the β-mannanase protein sequence via a peptide sequencer.

The amino acid sequences of the synthetic peptides were confirmed by sequence analysis using a protein sequencer PPSQ-21A (Shimadzu, Kyoto, Japan), and mass spectrometry using an AXIMA-CFR plus mass spectrometer (Shimadzu).

The full amino-acid sequence of the monomeric peptide was analyzed by sequential Edman degradation using a protein/peptide sequencer.

Sequencing was performed using a Genome Sequencer FLX using a GS Em PCR Kit II (Roche Applied Science).

The peptides were then sequenced using a Procise™ protein sequencer (Model 491 HT; Applied Biosystem, Warrington, UK) in the gas phase mode from Biobrene™-treated glass fiber discs.

High-throughput multiplex sequencing was carried out using a Genome Sequencer (GS) FLX Instrument (Roche Diagnostics, USA) and Genome Analyzer II (Illumina Inc., USA) sequencers.

Amino-acid sequence was determined by sequential Edman degradation using a protein/peptide sequencer at the Beijing Analyse Centre of Biomedicine (China).

Amplicon Sequencing was performed at 454 Life Sciences, Branford, Connecticut, USA, using a Genome Sequencer FLX.

AFLP fragment analysis using a capillary sequencer and intralane standardisation resulted in excellent methodical stability (≥ 98% similarity for GG and ≥ 94% similarity for CC).