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Peptides were desalted using a peptide microtrap (Michrom BioResources, Auburn, CA) and eluted using a 0.1% TFA, 95% ACN solution.
Following digestion, each fraction was desalted using a peptide microtrap (Michrom BioResources) according to the manufacturer's instructions.
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But an oral tablet form is also under development using a peptide drug delivery system developed by Enteris BioPharma.
We next addressed whether cleaved amyloid β peptides localize similarly to APP using a peptide-specific antibody.
Previously published peptide transport assays tend to follow peptide uptake using a radiolabeled peptide substrate.
These antibodies were generated in sheep using a nested peptide series conjugated to the metalloprotein, KLH.
Characterization of antibodies against histone PTMs used in the study was performed using a Histone Peptide Array (Active Motif, 13005).
All these data suggest that using a frameshift peptide array for cancer FS antigen screening is feasible and efficient.
Characterization of prorenin activation using a synthetic peptide substrate.
Loss of endogenous mouse β-cells was confirmed by measuring serum mouse C-peptide using a Mouse C-peptide ELISA (ALPCO Diagnostics; 80-CPTMS-E01 80-CPTMS-E01 80-CPTMS-E01
We have now used a related peptide, pH (low) insertion peptide (pHLIP), to translocate cargo molecules attached to its C terminus across the plasma membranes of living cells.
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