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Using a peptide library for LmSTI1a, we identified at least two distinct CD4 T cell mimetopes in each MHC class II haplotype, consistent with the induction of broad immunity.
Using a peptide library covering the full length of the HA protein, we found a 15-mer peptide that was able to induce T cell proliferation and IFN-gamma expression in chickens immunized with a fowlpox virus-based vaccine expressing the H5 protein.
Since no bona fide substrates for PCTAIRE-1 have been identified and the phosphorylation consensus sequence had not been defined, we recently performed positional scanning using a peptide library.
Using a peptide library approach, it has been shown that LKB1 only phosphorylates threonine residues, and strongly prefers a leucine two residues N-terminal to the threonine (i.e. at P−2) [ 33]. Figure 1 shows that leucine is present at P−2 in all ARKs and in CaMK1-α and -β, although in CaMK1-δ, CaMKI-γ and CaMKIV, the residue at P−2 is methionine.
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Biopanning using a peptide phage display library identified a peptide able to transduce heart tissue in vivo efficiently and specifically.
Using a random peptide library, a peptide sequence could be identified, against which 84 out of 90 SSc patients displayed antibodies.
The strategy using a designed peptide library shows real promise as a peptide-based high-throughput screening system.
However, using a combinatorial peptide library scanning approach, we identified a CDH13-derived peptide activating the 7B5 T cell clone.
HAMLET binding sites were identified using a synthetic peptide library covering most of the α-actinin-4 sequence (each peptide was 20 amino acids long, with a 5 amino acid overlap).
Construction of a novel protein-detection system was carried out using a designed peptide library with fluorescent labels based on loop structures.
We have determined the substrate preferences of the ABA-activated kinase OST1 using a combinatorial peptide library screening strategy [47], [48].
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