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We report herein the development of an efficient fluorescence assay for serine/threonine kinases using a peptide array.
Epitope mapping was done using a peptide array covering the DBL4ε domain.
A minimal linear binding epitope derived from the SKIP protein could be determined using a peptide array.
The main advantage of using a peptide array system is the possibility for simultaneously screening thousands of binding events in biological and pharmaceutical research [ 6].
We would also like to stress the importance of selecting specific antisera, such as using a peptide array as employed here, to the success of this or any ChIP protocol using low input cell numbers.
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In our study, we determined the Saccharomyces cerevisiae Atg1 consensus phosphorylation sequence using a peptide array-based approach.
We used a peptide array to determine the structural features of the FEI ligand that were important for receptor binding.
We also use a peptide array to identify the tripeptide FEI as the minimal binding motif and to define the consensus sequence for this ligand.
Here we report the use of a peptide array to discover acetyltransferase-mediated crosstalks.
In this work, we have defined the OST1 phosphorylation site preferences using a combinatorial peptide array to predict OST1 targets at the genome scale in guard cells.
Overall cellular immune responses were measured using three pools of a peptide array, Influenza Virus A/New Caledonia/20/99 (H1N1) Hemagglutinin Protein.
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