Sentence examples for using a pcr strategy from inspiring English sources

The zebrafish grn1 cDNA was cloned using a PCR strategy (Additional File 1).

The cDNA obtained was used as a template to obtain coding sequences (CDS) of GS using a PCR strategy.

Excision of L5 was confirmed in pREP41-Cre transformants using a PCR strategy (BWP33F, BWP33R, and BWP246F), followed by Southern blot analysis.

Isolation of the gene sequence was achieved by using a PCR strategy with degenerated primers targeted to conserved regions of orthologous HCT sequences available.

Accordingly we phased the SNP genotypes by using a PCR strategy to selectively amplify the DNA from the chromosomal strand containing the ~11 kb deletion.

In addition, tandem duplication, such as that found in the OnTI genes, or tandem repeat sequences such as those found in OnFT and OMADS9, would render genome walking using a PCR strategy particularly difficult to perform (Table 3).

pENTRY- pgp-3wt and - pgp-3ΔF508 were created using a PCR fusion strategy using pENTRY- pgp-3 as the template.

The two amplicons were purified and fused using a PCR fusion strategy (Hobert 2002).

In addition, using a PCR-based strategy the genomic content of eight tRNA/tmRNA gene sites that serve as DNA insertion hotspots were investigated in 28 diverse environmental and clinical strains of K. pneumoniae.

SOFA-HDV ribozymes were constructed using a PCR-based strategy that included two complementary and overlapping oligonucleotides as described previously [29].

Two different combinatorial TEVp substrate libraries, in the form of XNLXFXG and XNLXFXX, respectively, where X is any amino acid, were constructed by using a PCR-based strategy.