When necessary, PCR products were purified using a PCR Purification Kit (Qiagen Inc., Valencia, USA).
The PCR products were purified using a PCR Purification Kit following the manufacturer's instructions (Sangon Biotech Co. Ltd ,Shanghai, China).
The PCR products were purified using a PCR purification kit (QIAquick, Qiagen) and sequencing was performed by Macrogen Inc., Seoul, Korea, utilizing ABI 3730 XL automated sequencers (Applied Biosystems, Foster City, CA).
After PCR amplification, PCR products were purified using a PCR purification kit (Bioneer, Daejeon, Korea) and sequenced using a PRISM BigDye Terminator Cycle Sequencing Kit Applied Biosystemss, Foster city, USA) according to manufacturer's instructions.
The PCR product was purified using a PCR purification column (Qiagen, Crawley, UK), and sequenced using Big-Dye terminator chemistry with the same primers as those used in the original PCR.
The PCR products (megaprimers) were purified using a PCR purification kit.
PCR products were purified by using a PCR purification kit (ZYMO Research), and 150 ng of purified PCR products were applied for direct sequencing by capillary electrophoresis (Agowa, Berlin, Germany).
The resultant cDNA samples were purified using a PCR purification kit (Qiagen) and diluted 1 in 10 in PCR-grade water.
The PCR products were checked by agarose gel electrophoresis and cleaned using a PCR purification kit (Macherey-Nagel, Germany).
The resultant cDNA samples were purified using a PCR purification kit (Qiagen, 28104) and diluted 1 in 10 in PCR-grade water.
Ligations of digested and overlapped fragments were performed using T4-ligase (NEB, USA) in four tubes and kept overnight; then, the four tubes were gathered and purified using a PCR purification kit (Tiangen, Beijing, China).
