Sentence examples for using a pcr protocol from inspiring English sources

PCR reactions were performed in 10 μl with aliquots of normal and tumor DNA using a PCR protocol consisting of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min for a total of 40 cycles.

This study describes a simple and specific molecular detection test for the PVYN-W variant using a PCR protocol based on the recombinant point within the HC-Pro/P3 region of PVYN variants (PVYNTN, PVYN-W).

Using a PCR protocol specifically designed to genotype this deletion [30], we found it was correlated with the reduction of HPRT expression (data not shown).

Juveniles were distinguished from adults based on plumage characters [65], and sex determinations made in the field were verified using a PCR protocol that identifies a gene on the W chromosome [66].

Using a PCR protocol described previously [ 23], each sample was run in triplicate, and β-actin was run in parallel to standardize the input DNA.

Where it was positive for Brucella species but negative for the other two, B. canis was tested using a PCR protocol targeting the omp2B gene as previously described [ 18].

Ponderosae (Fig. 1; Appendix 2) using a PCR multiplex protocol that integrates fluorescent labels during PCR (Culley et al., 2008; Culley et al., 2013).

Staphylococcal cassette chromosome mec (SCC mec) type was identified by using a PCR-based protocol (9 ).

Tail DNA was extracted from the progenies for genotyping analysis using a PCR-based protocol provided by the Jackson Laboratory.

1.5 μg of DNA were used for a PCR-free library preparation using the NEBNext DNA Library reagents with Nextflex-96 indexes (Bioo Scientific, Austin, TX) using a PCR-free protocol.

Strains used in this study are isogenic to SK1 and are listed in Supplementary file 1. Gene disruptions and tagging were carried out using a PCR-based protocol (Longtine et al., 1998).