Sentence examples for using a pcr procedure from inspiring English sources

The presence of each TE was tested in six pools of 8 12 North American strains (Table 1, rightmost column) using a PCR procedure custom-designed for each chimeric TE (see Methods for details).

Genomic DNA was genotyped by using a PCR-based procedure.

Briefly, a cDNA library was screened for putative homologs using a PCR-RACE procedure with primers designed to match conserved regions of GOT proteins from a range of species.

The Kv3.1 knockout (KO) mouse line was kindly provided by Dr. R. Joho at UT Southwestern Medical Center and has been maintained using a PCR-based genotyping procedure as previously described [ 37- 39].

To optimize PCR at various annealing temperatures, we evaluated each primer pair using a gradient PCR procedure.

Then, specific primers for the T-DNA and specific primers for the adaptor sequence were used to amplify the unknown flanking sequences using a conventional PCR procedure.

IGS sequences were amplified by using a nested PCR procedure (30 ).

For B. burgdorferi, a 941-bp region of the 16S-23S rDNA spacer was used as a target for amplification using a nested PCR procedure (12).

Amplification of viral genome was performed using a nested PCR procedure, and patient-derived HIV-1 RT fragment carrying K101Q/Y181C/H221 replaced the partner sequence (2843 nt-3485 nt, 643 bp) in pNL4-3 pol to construct the first clone as previously described [ 29].

Amplification of viral sequences was done in a 0.5-ml Eppendorf tube using an immunocapture PCR procedure described previously [ 16].

In addition we detected induction of miR-210-3p miR-210-3p miR-210-3pRT-PCR procedupon