Sentence examples for using a pcr machine from inspiring English sources

Washed specimens were transferred to 0.2 ml PCR tubes containing 100 µl of 10 mM citrate buffer (8.2 mM tri-sodium citrate and 1.8 mM citrate), and antigen-retrieved at 97°C for 30 minutes using a PCR machine.

Since the oligos have a strong propensity to form intramolecular hairpin-loop structures, we annealed the two oligos by mixing them at equal molar ratios and then renatured in a step-wise process using a PCR machine: 95°C×30 sec, 72°C×2 min, 37°C×2 min, and 25°C×2 min, followed by an incubation at 4°C.

Ligations were performed overnight using a PCR machine.

1.4.8 Digest at 37° for 15 20 min using a PCR machine and load on 1.5% agarose gel immediately.

Synthesis reactions were carried out using a PCR machine (TProfessionnalTrio; Biometra, Goettingen, Germany) in 0.2 mL tubes.

McClure's team used a PCR machine--which copies and amplifies scraps of DNA--to search for two viral sequences, one from XMRV and the other from a closely related virus.

Individual standards were prepared from gene specific PCR products generated using a conventional PCR machine, electrophoresed on an agarose gel and subsequently extracted and quantified.

This problem is overcome by using a thermocycler (PCR machine) programmed to ramp from ambient to 51 °C over 12 30 min, rather than seconds.

Extracted DNA was used for amplification with the appropriate primers (as described above) using a conventional PCR machine (Perkin Elmer) and sequenced to confirm the identity of the amplicon.

The following conditions were used for multiplex RT PCR: 95 °C for 10 min, 95 °C for 15 s, 59 °C for 15 s and 72 °C for 1 min, with a final elongation step at 72 °C for 10 min using a Tercik PCR machine (DNA-technology, Moscow, Russia).

Quantitative PCR was performed using an ABI-7900 PCR machine.