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Quality of cDNA was assessed by using a PCR control run with human β-actin.
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Detection of β-actin was used as a PCR control.
Also, a pair of primers for the amplification of a modified plasmid was used as a PCR control; this was essential to distinguish between an inhibited amplification reaction and a sample that contained no DNA.
We assessed the total RNA samples for quality control before using a PCR assay (Human Osteogenesis RT Profiler PCR Array; SABiosciences [formerly SuperArray Bioscience], Frederick, MD, USA).
An internal amplification standard (ITAS) was added to the PCR mixture in quantitative TRAP assays or was used independently as a PCR control.
A case-control study using a PCR-based method was performed to compare the allelic distribution of hTERT-VNTR2-2nd in DNA samples from cancer-free controls and patients with cancer.
Before cDNA synthesis the RNA purity (i.e. removal of DNA) was examined using a control PCR that amplifies the constitutively expressed gene PFY1 encoding Profilin.
Real-time PCR reactions for each miRNA (10 μl volume) included 4 μl of RT product, were performed in triplicate and included no-template and PCR controls using an ABI7900 PCR system (Applied Biosystems).
A buffer control was taken through each extraction procedure with no sample added and used as a PCR negative control in each PCR reaction to control for contamination.
Purified viral DNA from the EHV-2 LK strain was used as a PCR positive control.
None of the sequenced samples were identical with the type strain G37 used as a PCR standard control.
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