Sentence examples for using a pcr cloning from inspiring English sources
Poly-A treatment, insertion of the PCR products into pDrive vector, transformation of E. coli EZ competent cells (Qiagen) and cloning were performed using a PCR Cloning Kit (Qiagen) as described by the supplier.
Amplified cDNA fragments were isolated by agarose gel electrophoresis, purified by NucleoSpin Extract II (Macherey-Nagel, Germany) and cloned using a PCR cloning Kit (Qiagen, Germany).
After five rounds of affinity selection, the RT-PCR product was cloned using a PCR cloning kit (Qiagen) and sequenced with an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Foster, CA, USA).
The amplification was carried out under the following conditions: denaturation at 94°C for 5 min, 30 cycles of (94°C for 1 min, 57°C for 1 min, 72°C for 1 min), and a final extension at 72°C for 10 min. The PCR products were cloned using a PCR Cloning Kit (QIAGEN).
After gel-electrophoresis analysis, the second-round PCR products were directly cloned using a PCR-TA cloning kit (pCRII TOPO, Invitrogen, Carlsbad, CA, USA).
The zebrafish grn1 cDNA was cloned using a PCR strategy (Additional File 1).
PCR products were purified using a PCR purification kit (Qiagen) and cloned into the pBS-TgDHFR vector that encompasses polylinker sites flanking the modified Toxoplasma gondii dihydrofolate gene (tgdhfr/ts) conferring resistance to pyrimethamine (Dessens et al., 1999).
For clonal sequencing, the nested gel-purified NS3 or NS5A PCR products were cloned into the pJET1.2/blunt cloning vector using a CloneJET PCR cloning kit (Fermentas, Glen Burnie, MD), while NS5B was cloned into the GT1a or GT1b replicon shuttle vector cassette as described previously (10, 11, 13, 15, 16).
These fragments were cloned using a StrataClone PCR Cloning Kit (Agilent Technologies).
The purified PCR product was cloned into pJET1.2 by using a CloneJET PCR Cloning Kit (Fermentas) according to the manufacturer's recommendations.
Amplified fragments were cloned into vector pSC-A using a Strataclone PCR cloning kit (Agilent Technologies cat.no. 240207).
