Sentence examples for using a pcr assay from inspiring English sources

Samples that yielded PCR products were confirmed by using a PCR assay incorporating the 120-M59´ and 120 807´ primer pair, which amplifies a 833-bp fragment of the ompB gene of Rickettsia, as previously described by Roux and Raoult (13 ).

Available isolates were identified as C. difficile using a PCR assay to detect the presence of gluD and were PCR ribotyped as previously described [ 8, 13].

Samples were tested using a PCR assay designed to amplify a full-length major piroplasm surface protein (MPSP) gene of a cervine Theileria species, designated as Theileria sp. (sika 1).

At various times after galactose addition, aliquots were removed and genomic DNA was isolated to analyze for the formation of a DSB at the MAT locus using a PCR assay.

Using a PCR assay for analysis of Septin 9 (SEPT9) hypermethylation in DNA extracted from plasma, clinical performance was optimized on 354 samples (252 CRC, 102 controls) and validated in a blinded, independent study of 309 samples (126 CRC, 183 controls).

We verified the presence of this deletion using a PCR assay according to [ 29].

The authors of this study used a PCR assay for the mouse intracisternal A particle (IAP) which they reported to be more sensitive than a mouse-specific D-loop mtDNA-based assay [26].

We used a PCR assay to query the presence/absence of the BPSS0654 taurine dioxygenase gene across large B. pseudomallei strain collections from Thailand and Australia.

We completely sequenced the control region (D-Loop) from each of these samples and used a PCR assay to assess the presence of the 4977 bp common deletion.

Because of the limited amount of DBS material available, we used a PCR assay that detects sequences from all 4 major PTLV groups.

The Sxr b junction fragment was confirmed using a PCR RFLP assay (Fig.  5C).