Sentence examples for using a pcr approach from inspiring English sources

Typing of SCCmec elements of types I to IV was carried out for one isolate of every spa type from every farm using a PCR approach, including a combination of different PCRs [ 35].

Using a PCR approach, the cloned gene (PyElo) possessed an open reading frame (ORF) of 834 bp encoding 277 amino acid residues.

We have cloned rFTR1 by using a PCR approach relying on degenerate primers designed from the conserved regions of Saccharomyces cerevisiae high affinity iron permease.

These alleles were later confirmed using a PCR approach [ 13].

We then discriminated between deletions and Ty1 insertions events using a PCR approach.

In this report, using a PCR approach, we identified the ndh genes in different Oncidiinae plants.

The N-terminal 3×FLAG tag was added to pENTRY- pgp-3wt and pENTRY- pgp-3ΔF508 using a PCR fusion approach.

The ATF4-dEGFP reporter was generated using a PCR fusion-based approach.

By using a PCR-based approach, fragments of genes coding for H+-PPases in a number of protists, both free-living and parasites of animals and plants, that belong to diverse taxonomic groups (trypanosomatids, ciliates, apicomplexans, euglenoids, amoeboid mycetozoa, heterokonts) have been isolated.

Fragments of EcR and USP were cloned from two insect cell lines, Sf21 and High Five™ cells (derived respectively from Spodoptera frugiperda and Trichoplusia ni), using a PCR-based approach employing degenerate primers designed on the basis of conserved regions of nuclear receptors, together with 5′- and 3′-RACE.

Mice were genotyped using a PCR-based approach to identify the DICER+/− and DICER−/− mice.