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Purified genomic DNA was used as a template to detect the remaining episomal vectors in the hiPSCs using a PCR analysis.
TG pups were identified using a PCR analysis of tail biopsies taken after weaning.
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Using a PCR assay for analysis of Septin 9 (SEPT9) hypermethylation in DNA extracted from plasma, clinical performance was optimized on 354 samples (252 CRC, 102 controls) and validated in a blinded, independent study of 309 samples (126 CRC, 183 controls).
This approach permits the biologist to experimentally confirm the insertion point using a PCR-based analysis.
Since chromosome loss would result in LOH for markers on both centromeric arms, we examined Ura− derivatives using a PCR-based analysis to detect LOH for markers on both the right and left arms of chromosomes III or V (details in File S1).
Polymorphisms in BER genes were analysed using a PCR with high-resolution melting analysis.
A statistical analysis using a pCR definition that also includes cases with residual in-situ carcinoma yielded similar results (not shown).
The present study was carried out to determine the distribution of gene polymorphisms using a PCR-based restriction analysis.
Copy number changes determined using our VN analysis were confirmed using a PCR-based copy number analysis approach.
The loss of episomal vectors was confirmed using a genomic PCR analysis (Figure S3A). Figure 2: Establishment of iPS cell clones under the feeder-free (Ff) and xeno-free (Xf) culture system.
All mice were maintained on a CD1 background, and genotyped using a standard PCR analysis as previously described.
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