Sentence examples for using a pcr amplification from inspiring English sources

We attempted to validate some of these gain genes using a PCR amplification method.

All PCR reactions were performed by using a PCR amplification kit (Takara Biomedical, Otsu, Japan) according to the manufacturer's instructions.

All PCR reactions were performed by using a PCR amplification kit (Takara Biomedical) according to the manufacturer's instruction.

Kozarewa et al. [ 24] demonstrated that using a PCR amplification-free enrichment step that relies solely on cluster amplification on the sequencing platform for the enrichment of the library, resulted in a more uniform distribution of reads.

However, this method uses a PCR amplification step which may introduce bias against long transcripts, thus selecting for partial (shorter) transcripts.

These two primers were used in a PCR amplification with Pwo polymerase (Roche), using the pGEM-T Easy clone of the intron-containing promoter as template.

The procedure for global mRNA amplification can be performed either linearly, using T7-based in vitro transcription [ 2- 4] or exponentially, using a PCR-based amplification [ 5] or a combination of both [ 6].

He has developed a technique for full genome microarray analysis on RNA amounts equivalent to that from a single cell using a PCR-based amplification step.

The eluate was quantified using a Qubit fluorimeter and 20 ng of this product was used in a PCR amplification with 20 μL Phusion Master Mix (NEB), 5 μL of 10 μM modified Solexa© Amplification primer mix (Illumina, Inc).

5 µL of this product was used in a PCR amplification with 50 µL Phusion Master Mix (NEB), 5 µL of 10 µM modified Solexa© Amplification primer mix (2006 Illumina, Inc., all rights reserved; P1-forward primer: 5'-AATGATACGGCGACCACCGA-3'; P2-reverse primer: 5'-CAAGCAGAAGACGGCATACGA-3'), and 40 µL H2O.

MCPyV was detected by nested PCR by using a first PCR amplification with the LT1 and VP1 primer sets published by Feng et al. (2 ).