Sentence examples for using a pcDNA3 vector from inspiring English sources

Two bicistronic constructs expressing shRNA for uPAR/cathepsin B (pUC) and uPA/uPAR (pU2) were constructed using a pcDNA3 vector as described previously by our group [5].

Bicistronic constructs expressing siRNA for uPAR/cathepsin B (pUC) were constructed using a pcDNA3 vector as described previously by our group, and all vectors were expressed under the control of a CMV promoter.

The small insert library was constructed using a pUC18 vector (http://www.jgi.doe.gov/sequencing/protocols/prots_production.html).html

Bovine Hsc70 (1 554) with a C-terminal His-tag was expressed in Escherichia coli at 25°C, using a pET21a vector.

Bovine auxilin (547 910) was expressed as a GST fusion protein in E. coli at 25°C, using a pGEX4T-1 vector.

Lentivirus expressing a short hairpin RNA (shRNA) specific for CD82 or GSK3 β were constructed using a pLKO.1 vector backbone (AddGene, Cambridge, MA, USA).

The contributions of the four ancestral populations to an individual's genome can be represented using a 4D vector whose components sum to 1.

The mouse Rassf7 probes were generated using a pCMV.sport6 vector (IMAGE clone 4206694) and an SP6 (sense) or T7 polymerase (antisense) following digestion with StuI or AhdI enzymes respectively.

The ETvmat2 green fluorescent protein (GFP) transgenic zebrafish was identified from a large-scale enhancer trap screen using a Tol2 vector containing a 249-bp zebrafish gata2 minimal promoter linked to a GFP reporter gene [ 18, 19].

Then, we evaluated the effect of the ERSE motif's direction on the responsiveness of the mouse CRELD2- ALgenegene pair to Tg by using a pGL3 vector containing the SV40-promoter.

PBMC samples were obtained from vasculitis patients (50 patients in total) regardless of the patient's symptom, active, or inactive phase, and a cDNA library was prepared from their pooled mRNAs by the linker-primer method using a pAP3neo vector.