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Chloroplast transformation is carried out using a particle bombardment device as schematised in Figure 1.
a) Introduction of foreign DNA material into the chloroplast of C. reinhardtii is carried out using a particle bombardment device.
Ito and Fukuda ([2002]) achieved the transformation of cultured Z. elegans cells with the Z. elegans endonuclease 1 (ZEN1) gene using a particle bombardment method.
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Proliferating embryogenic tissues were subjected to transformation through particle bombardment using a particle inflow gun (PIG) [ 93] following the procedure of Droste et al. [ 92] or using the combined methods of DNA-free particle bombardment and Agrobacterium transformation [ 94].
The third system to introduce DNA is formed by a particle bombardment DNA delivery system.
Stable nuclear transformation of P. morum using the particle bombardment technique is now feasible.
Both the reporter and effector vectors were co-transformed into tobacco leaves using the particle bombardment.
Transient TRY expression analysis was carried out by using the particle bombardment method in Arabidopsis cotyledons [ 47].
Both of the reporter vectors and their corresponding effector vectors were co-transformed into tobacco leaves using the particle bombardment.
GFP and RFP fusions proteins were expressed in fava bean (Vicia faba) and onion (Allium cepa) epidermal cells layers using the particle bombardment technique PDS-10000, BioRad) [ 59].
The pUT2 and pG418-T1A plasmids were then used to co-transform strain M4 using particle bombardment.
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