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The localized measurement of pH values inside the polymer matrix was performed, using a pH sensitive nitroxide (pKa 6.1).
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The layer growth on gold electrode resonator was monitored with QCM and the catalytic activity of the enzyme multilayers was studied with UV Vis spectroscopy, using a pH-sensitive dye (bromocresol purple).
Thus, we evaluated the effects of targeted delivery of SDF-1α using a pH-sensitive polymer poly (urethane amino sulfamethazine) (PUASM), a synthetic macromolecule with potential for targeted drug delivery in acidic conditions, to enhance therapeutic neurogenesis and angiogenesis in a rat model of permanent middle cerebral artery occlusion.
To investigate whether CFTR is involved in HCO3− transport in Sertoli cells, we measured intracellular pH using a pH-sensitive fluorescent probe BCECF-AM.
The intracellular pH of S. thermophilus was measured at external pH values of 6.0 to 9.0 using a pH-sensitive fluorescence probe 5 (and 6- -carboxyfluorescein succinimidyl ester (CFSE) as described by Sawatari and Yokota [28], which was based on the method originally described by Breeuwer et al. [29].
Resulting changes in islet pHi were monitored by confocal microscopy using a pH-sensitive fluorescent indicator.
The hydrolysis of most carboxylate esters was monitored using a pH-sensitive colorimetric assay.
The hydrolysis of lactones was monitored using a pH-sensitive colorimetric assay.
Dox/DPPE-GA lipid conjugation was accomplished using a pH-sensitive hydrazone chemistry to provide controlled drug release.
Using a pH-sensitive fluorescence-labelled probe (LysoSensor DND-189, Molecular Probes, Eugene, OR, USA), a reduction in lysosomal pH and intensity of lysosomal staining was detected in KB-CP-r cells as compared to the KB-3-1 cells.
Using a pH-sensitive fluorescent probe and fluorescent microscopy applied to a solid tumour growing in a transparent window, Helmlinger et al (1997) demonstrated a gradual decrease of pHe from 7.4 to 6.7 as the distance from blood vessels increased from 0 μ M to 200 μ M.
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