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pH was determined using a pH electrode which was calibrated with two standard buffer solutions of pH 4.0 and 9.2.
The pH value was measured directly using a pH electrode Sen Tix 41 (WTW, Germany) in aqueous extracts.
The suspension pH was measured using a pH electrode (Metrohm, 6.0234.100) and readjusted, if necessary, to pH 6.0 ± 0.05 by small additions of NaOH or HCl.
The sensing scheme is based on the pH change occurring in a low buffer system by the GST reaction, which is measured potentiometrically using a pH electrode.
For pH measurement, 10 g of soil was suspended in 25 ml of a 0.01 molar CaCl2 solution for 1 h, stirred twice and pH determined using a pH electrode (VDLUFA standard method A 5.1.1).
Another 5 mL algal solution was sampled and centrifuged each day to allow the solution's pH value to be determined (at the same height in each suspension) using a pH electrode connected to a pH meter (PHS-3C, Shanghai LIDA Instrument Factory, China).
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pH was determined at 25 °C by weighing 1 g portions, pulverizing with a wooden mallet, hydrating with distilled water (1 2.5 g/v), and using a pH meter (Orion pH electrode 9165 BN, Orion Research, Boston, MA, USA) to take measurements.
Under continuous stirring, 100 μL of 0.10 mol.L-1 sodium hydroxide solution was added, then allowed to equilibrate, and the pH recorded using a pH meter with a glass electrode (Quimis, Diadema, Brazil).
However, monitoring the real-time acidification profile of the oxygen scavenging system in the chamber is inaccessible to the conventional methods of using a pH paper or common laboratory electrode.
pH was measured using a pH meter fitted with a glass electrode as described by Nunes and Emond (1999).
The effluent and influent pH values were measured using a pH metre and a combination glass electrode calibrated in buffers at pH 7 and 9.
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