Sentence examples for using a pGEX vector from inspiring English sources

In this work, the glucagon peptide was expressed in a soluble form using a pGEX vector in a bacterial system, yielding a high concentration of the purified product.

A human FAPP1-PH construct containing a C94S substitution was expressed in Esherichia coli by using a pGEX-6P-1 vector (Amersham Biosciences, Piscataway, NJ, USA) in M9 media supplemented with NH4Cl and C6-glucose.

Full length human RIα was purified using a pGEX-KG/RIα construct as reported [18].

Cloning into a pGEX vector allowed the expression as GST fusion proteins.

Rabbit CRT domain-deletion mutants were constructed in a pGEX vector.

Rabbit anti rat GRASP65: bacterially expressed rat N-terminally tagged GST-GRASP65 (from a pGEX vector) was injected in rabbit.

In this work, the glucagon peptide was expressed and purified from Escherichia coli cells using the pGEX vector system.

GST fusions of JNK, p38, c-Jun (1-79) ATF2ATF2 (1-109) were expressed in E. coli using pGEX vectors and purified by glutathione agarose affinity chromatography as described previously [21].

A library of randomly fragmented ifp genes was constructed as illustrated in Figure S8 using pGEX2TK-IFP1.4, a pGEX-2TK-derived vector that expresses IFP1.4 from a tac promoter with glutathione S-transferase (GST) fused to its N-terminus and a hemagglutinin (HA) tag fused to its C-terminus.

All vector backbones were gifts of Dr. Aaron Straight and are described in Figure 1 figure supplement 2. ExonC and the C-terminal 50 residues of mouse Spire1 were cloned into the avi-his-MBP-TEV-3xFLAG-precision vector described above or a modified pGEX vector (for N-terminal GST fusion proteins) using standard techniques.

The DNA fragment encoding Fluc was obtained by PCR using pGEX-Ppy vector [ 23] as a template, and primers LucNotG4SB (5′- gg cgc gcc GCG GCC GCC GGT GGT GGT GGT AGC ATG GAA GAC GCC AAA AAC ATA AAG-3′) encoding a G4S linker and NotI site (underlined) and LucXhoF (5′- g gcg cgc CTC GAG CTT TCC GCC CTT CTT GGC CT- 3′) containing XhoI site (underlined).