RT-PCR was performed using a OneStep RT-PCR kit (QIAGEN) as per manufacturer's instructions.
To assess PHD3 and GAPDH expression, 500 ng of total RNA was used for reverse transcription using a OneStep RT-PCR Kit (Qiagen).
The RT-PCR assay was carried out using a OneStep RT-PCR Kit (QIAGEN).
Reverse transcription polymerase chain reaction was then performed using a OneStep RT PCR Kit (Qiagen).
RT PCR was carried out using a OneStep RT PCR kit (Qiagen) on 25 ng of total RNA.
Genomic fragments were amplified by using a OneStep RT-PCR Kit (QIAGEN, Hilden, Germany) as described by the manufacturer.
The isolated HIV-1 env RNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using a OneStep RT-PCR Kit and HotStar Taq DNA Polymerase (QIAGEN , Inc.
First-strand cDNA was synthesized from the total RNA using a Onestep RT-PCR kit (Qiagen), Ready-To-Go You-Prime First-Strand Beads (GE Healthcare Bioscience) and 50 ng of random primers (Invitrogen) as recommended by the manufacturer.
Reverse transcription and first-round amplification of the capsid protein (VP1)/2A junction region of HAV were performed by using a Onestep RT-PCR Kit (QIAGEN) and primers (Table 1).
The complete coding regions of both the HA and NA genes of the strain A/Swine/Finland/si723/09 were amplified in three overlapping parts by using a OneStep RT-PCR Kit (Qiagen, Hilden, Germany).
