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RT-PCR was performed on total RNA as described previously using a One Step RT-PCR Kit following the manufacturer's protocol (#210210, Qiagen) [ 18].
RT-PCR was performed using a One Step SYBR PrimeScript PLUS RT-PCR Kit (Takara Bio Inc.).
Magnesium borate hydroxide (MBH) nanowhiskers were synthesized using a one step hydrothermal process with different surfactants.
For strand-specific RT-PCR, RNA was purified as previously described [48] and subjected to RT-PCR using a One Step RT-PCR kit (Qiagen).
RT-PCR was performed using a One –Step RT-PCR kit (Clontech, Mountain View, U. S. A). according to the manufacturer's instructions.
The RT-qPCR assay was performed using a One Step RT-PCR kit (Qiagen).
Similar(48)
Plasma GDF-15 was measured using a one-step enzyme immunoassay based on the electrochemiluminescence (ECLIA) technology (Elecsys 2010).
Hematite core-shell nanoparticles with plate-like morphology were synthesized using a one-step hydrothermal synthesis.
Mn3O4 graphene (Mn3O4–GR) hybrids were synthesized using a one-step strategy under solvothermal conditions.
The assay detected 32 isolates of 15 definite and 2 tentative ilarvirus species using a one-step RT-PCR.
They make it using a one-step process, as opposed to by using the multilayer lithography techniques employed to fabricate integrated circuits.
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