Sentence examples for using a nucleotide blast from inspiring English sources

The 16S rDNA sequences were used to search the GenBank database using a nucleotide blast algorithm (http://blast.ncbi.nlm.nih.gov/) to display the closest matches to the 16S rDNA sequences for known species.

Primers were then designed for 15 microsatellite markers using Primer3 software (http://frodo.wi.mit.edu/primer3) and checked for the presence of SNPs using the SNPCheck bioinformatics program from UK National Genetics Reference Laboratory (https://ngrl.manchester.ac.uk/SNPCheckV2.1/snpcheck.htm) and for human ALU repeats using a nucleotide BLAST search (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi

In the present study, we analyzed bacterial 16S rDNA sequences using a standard nucleotide BLAST search of GenBank for homology with known bacterial 16S rDNA sequences.

The sequenced clones were analyzed using a standard nucleotide BLAST search of GenBank for homology with known bacterial 16S rDNA sequences.

The Arabidopsis thaliana GTS1 cDNA sequence (AT2G47790) was obtained from the Arabidopsis-TAIR website and used to perform a nucleotide BLAST (BLASTn) search of the Oryza sativa (rice) genomic sequence on the SALK Institute RiceGE2 web interface.

A nucleotide BLAST using the primers from the LINE-1 element and the VP35-like region as queries, and the Myotis lucifugus genome assembly (WGS) as a database, yielded only a single match to the targeted region of cont2.261 (AAPE02000262).

A nucleotide BLAST search using the 256 bp flanking sequence revealed that this sequence mapped to chromosome 16 B2, consistent with the FISH localization (Fig. 1D).

In addition, the presence of genomic clusters in the D. solani and D. dadantii species was searched with a nucleotide BLAST analysis using the sequences of the specific clusters as queries against a database constituted by the D. solani and D. dadantii genomes (e-value threshold = 10-50).

The resulting sequences were compared to GenBank using a BLAST program: the nucleotide blast.

Because PlantTribes 2.0 includes the Physcomitrella patens version 1.1 gene annotations from Phytozome [ 66], we used a nucleotide BLAST+ search of a local database of Physcomitrella patens version 1.6 annotated coding sequences to identify the current gene annotations for ease of reference (Additional file 2, which includes all of the genes used in this paper).

Sequence identification was performed using NCBI nucleotide BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi