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Moreover we applied a slightly different image analysis approach than the in Figure 1 by using a nuclear staining as a mask for the Jacob channel and thereby excluded the nuclear membrane from the analysis.
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Nuclear fragmentation, which is observed during apoptosis, is observed using a nuclear stain DAPI.
Apoptosis of the colonic epithelium was assessed using a nuclear stain, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma, USA).
Along this line, Strijbos et al (2010) used a nuclear stain to enumerate CECs in prostate cancer patients receiving Docetaxel.
Appropriate Alexa Fluor® 488-conjugated secondary antibodies were used, and DAPI (4′,6-diamidino-2-phenylindole) was used as a nuclear stain.
Propedium iodide was used as a nuclear stain.
4, 6-diamino-2-phenylindole (DAPI) was used as a nuclear stain.
Hoechst 33 342 was used as a nuclear stain (Molecular Probes, Life Technologies R376055).
(d) Immunofluorescence of MEF2D (red) in hippocampal slices (Dapi (4',6-diamidino-2-phenylindole) was used as a nuclear stain, insets shown zoomed images of area outlined).
The fluorescent ABCB1 substrate doxorubicin (10 μM) was added for a further 1 h, to assess doxorubicin efflux in the presence of Act D. Hoechst 33342 was used as a nuclear stain once cells had been formalin fixed and permeabilised with 0.1% Triton-X.
WGA has specificity for N-acetylhexosamines or sialic acid in certain linkages, and while there is some tissue reactivity with WGA [ 14], this lectin is primarily used as a nuclear stain in fly embryos and does not react with the PM, for example [ 26].
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